Composite

Part:BBa_K4408007:Design

Designed by: Leyu Xu   Group: iGEM22_Worldshaper-NJBIOX   (2022-09-29)


Lipase CALB connected with chitin binding domain


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 949
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 670
    Illegal NgoMIV site found at 780
    Illegal AgeI site found at 472
    Illegal AgeI site found at 730
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1025
    Illegal SapI site found at 964
    Illegal SapI site found at 994


Design Notes

We used CALB enzyme to synthesize butyl butyrate from butanol and butyrate. By fusing ChBD to CALB enzyme, CALB can be purified efficiently through chitin purification. ChBD-fusion CALB can enhance the reaction rate of CALB catalyzed esterification.


Source

Linker 2 is based on part BBa_K4408004. CALB is based on part BBa_K2302006. ChBD is based on part BBa_T2028.

References